Coding

Part:BBa_K863020:Design

Designed by: Isabel Huber   Group: iGEM12_Bielefeld-Germany   (2012-09-19)

bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 190
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers for isolation of the gene with BioBrick Prefix, pT7 promoter and RBS in the fwd primer and Suffix and His-tag in the rev primer.
fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGAGtaatacgactcactatagggagagaggagaaaaATGAAAAAATCATATGGAGT-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTATTATTAGTGATGGTGATGGTGATGCTCAGGCATATTTGGAA-3'

Source

The gene sequence was isolated from Bacillus halodurans C-125.

References

Ruijssenaars, H. J. and S. Hartmans (2004). "A cloned Bacillus halodurans multicopper oxidase exhibiting alkaline laccase activity." Appl Microbiol Biotechnol 65(2): 177-182.